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From left to right: Hunter Christensen, Kristin Lewis, Trinity Clifford and Ahmad Crawford are pictured here on the Lewes campus. They spent their summer on the University of Delaware’s Hugh R. Sharp Campus in Lewes, conducting research to help determine different influences and variables on metabolic rates in Clearnose skates.
From left to right: Hunter Christensen, Kristin Lewis, Trinity Clifford and Ahmad Crawford are pictured here on the Lewes campus. They spent their summer on the University of Delaware’s Hugh R. Sharp Campus in Lewes, conducting research to help determine different influences and variables on metabolic rates in Clearnose skates.

Clearnose skate metabolism

Photos by Audrey Tong

Undergraduate students spend summer in Lewes, Delaware studying Clearnose skates

This summer, four undergraduate students teamed up at the University of Delaware to study one of Delaware’s most abundant aquatic creatures: the Clearnose skate. 

The Clearnose skate is Delaware’s most common skate, a flat, ray-like fish found in the Atlantic Ocean, the Delaware Bay and the Delaware Inland Bays. However, just because it’s a common fish doesn’t mean every aspect of the skate has been thoroughly studied. Much is still unknown about the skate’s oxygen intake and metabolism.  

To help determine different influences and variables on metabolic rates in Clearnose skates, four students—one participating in this year’s Research Experience for Undergraduates (REU) with funding from the National Science Foundation, one participating in the Doris Duke Conservation Scholars program, which U.C. Santa Cruz administers, and two participating in the University of Delaware Summer Scholar’s programs—spent the summer investigating different aspects of their metabolic patterns. 

The research took place under the guidance of Aaron Carlisle, assistant professor in UD’s School of Marine Science and Policy (SMSP), and members of his lab group. 

The REU participant was Kristin Lewis, a rising junior studying biology at Howard University. The Summer Scholars were Trinity Clifford and Hunter Christensen, both rising seniors studying marine biology at UD. Ahmad Crawford, a marine science major at Eckerd College in Florida, participated thanks to the Doris Duke Conservation Scholars program. 

Working for 10 weeks over the summer at the Hugh R. Sharp Campus in Lewes, the students used different groups of skates for their research. They performed respirometry experiments on all of them—looking at how much oxygen the fish used while in the tanks—and then compared the skates’ metabolic rates. 

Students spent their summer conducting research to help determine different influences and variables on metabolic rates in Clearnose skates.
Students spent their summer conducting research to help determine different influences and variables on metabolic rates in Clearnose skates.

Lewis looked at what the lab has called the “couch potato effect,” to see how time in captivity affects the physiology of skates. 

“We're essentially looking at whether the animals we've had in captivity have become lazy, or gotten out of shape because they've been here so long,” said Lewis. “We're comparing the metabolic rates of skates that we've had for many months with skates we've just recently captured.” 

Lewis hypothesized that skates held in captivity will have lower metabolic rates than wild skates. She is currently analyzing her data to determine if holding time impacts metabolism in skates. 

Crawford looked at two factors on metabolic rates: body size and the sex of the skates. He said that with respect to metabolic rates across other species, females tend to have higher metabolic rates, so he wanted to see if that trend was the same for skates. 

For body size, Crawford explained, “We want to know if smaller animals are going to have a higher mass specific metabolic rate than bigger animals. We're trying to see if that trend is the same for Clearnose skates.” 

Christensen looked at the digestion of the Clearnose skates. He explained that they fed the skates right before testing their metabolic rates which gave them a sense of how much energy they use in digestion. More specifically, he looked at how different diets affect the skates’ metabolism. 

“Different prey items vary in the amount of macronutrients they have,” said Christensen. “We'll feed them shrimp which is very high in protein and then we’ll feed them sardines which is very high in fat and that should hopefully show a difference in the amount of energy they need to use to digest it.” 

Clifford looked at how the diel cycle affects the skates and hopes to find evidence of a circadian rhythm—a pattern of behavior that occurs every 24 hours such as a sleep cycle.

“I was interested to see if they were more active at night or more active during the day,” said Clifford. “We do this using respirometry data where we seal the skates in a giant tank, watch their oxygen consumption rates, and we equate that to energy use. If they're consuming more oxygen, we believe they're more active or using a lot more energy, and if they're lower, they're less active or using less energy.”

Throughout the summer, the four students bonded through their shared research experience, especially since much of what they studied hadn’t been thoroughly researched before. 

Kristin Lewis (left) and Trinity Clifford measure a Clearnose skate as part of their summer research
Kristin Lewis (left) and Trinity Clifford measure a Clearnose skate as part of their summer research.

“There's not a lot of research on skates and respirometry so we were kind of learning how to do it ourselves. We eventually figured out the system but when we started, there were a lot of different problems we had to work through,” said Lewis. “We grew to figure out ways to do each experiment and how to better work on everybody's experiment and that's just an incredible amount of knowledge we gained.” 

In addition, they pointed out that they had help from Carlisle and graduate students in Carlisle’s lab. Getting a chance to interact with those students helped them to realize that they had made the right choice when it came to an area to study in school.  

“The grad students and all the other people who've helped us along the way have been so great. It’s been amazing to have these people that we can rely on, this backbone that we have because I don't think we could have done this without the people in our lab,” said Lewis. “Seeing them and interacting with them, I'm looking forward to going to grad school because I see all that they do and I'm just like, ‘This is the path I know that I want to be on.’ It ensures that I'm going in the right direction.” 

Christensen agreed with those sentiments, adding, “We couldn’t have accomplished as much in the summer if it weren't for the grad students. They have been very hands-on and helped us so much.” 

Tess Avery, one of the graduate students in Carlisle’s lab who helped with the students’ projects, said they have all been incredible to work with. 

“It's been so much fun for me to work with them,” said Avery. “I also am not someone that has worked with respirometry before, so I learned a lot of it along with them, which has been an interesting experience for me as they're learning. They're doing an amazing job doing their work independently. They’re curious about it and it's been really great.” 

Having come to UD from outside institutions, both Crawford and Lewis said they were a little nervous at first, but those nerves dissipated almost the instant they got to UD. 

“Once I got here, everyone was very accepting of you and just there to help you anytime you needed it,” said Crawford. “They were always there to give you a new experience to learn so you could learn new skills and make you a better marine science student. It’s been a really good experience the entire summer.” 

Crawford said in addition to all the research, the students were also able to learn a lot about animal husbandry, something he hadn’t had much experience with before this summer. 

“I never really knew how to take care of wild animals,” said Crawford. “Being able to feed them and do water changes, and water quality tests, just how to take care of the animals and keep them alive was a good skill to learn.” 

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